Standardization and validation of an alkaline phosphatase ...

Standardization and validation of an alkaline phosphatase-linked immunoassay to quantify a plant-derived antibody directed against the hepatitis B virus surface antigen. Leyva A(1), Hernández N, González T, Sánchez JC, Franco A, Delgado I, Montañez M, Valdés R.

Use of p-nitrophenyl phosphate for assay of soil ...

A simple method of assaying soil phosphatase activity is described. It involves colorimetric estimation of the p-nitrophenol released by phosphatase activity when soil is incubated with buffered (pH 6·5) sodium p-nitrophenyl phosphate solution and toluene at 37° C for 1 hr.The method is rapid and precise, and it has significant advantages over methods previously proposed for assay of soil ...

PHOSPHATASE ESTIMATION DETERMINATION LIBERATED

Alkaline Phosphatase.-Comparisons between these two phosphate procedures, onthe onehand, andthe phenol method of determining phosphatase on the other, have yielded results which agree very well. These are set out in Tables I and 11. It is apparent that with phenylphosphate as substrate the sameresult, or nearly the

Estimation of serum alkaline phosphatase activity

There are two type of phosphatase,i.e. 1. Alkaline phosphatase with a maximum activity at about pH 10 2. Acid phosphatase, maximum activity between pH 5 and 6. 3. Alkaline phosphatase in serum acts on the substrate disodium phenylphosphate buffered at pH 10 at 37 ºC to liberate phenol.

The use of alkaline phosphatase and possible alternative ...

CAC stated that 'The level of alkaline phosphatase is decreased with heat, such as at temperatures typically applied in separation and in thermization'. Although thermised milk is ALP-positive, the temperatures used for thermisation (57–68°C) can lead to some reduction in ALP activity, but the time is too short (5–20 s) to establish a ...

ENZYMATIC REACTIONS OF ALKALINE PHOSPATASE | The …

Alkaline phosphatase is a ubiquitous enzyme that can be isolated from bone, kidney, intestine, plasma, liver, spleen, plants and microorganisms (Sakharov et al,1998). The function of alkaline phosphatase in enzymatic reaction is to remove phosphate that attached to an alcohol in a substrate. The product from this reaction will be a free ...

Estimation of alkaline serum phosphatase in theory and ...

Methods of estimating alkaline phosphatase in serum and the value of different units are discussed. A modification of Bodansky's original method was evolved in which the serum was incubated in a veronal and NaOH buffer at p H 9.3 and the phosphatase activity was measured by the amount of phosphate split off from a standard substrate of Na-ss-glycerophosphate.

Alkaline phosphatase activity of a phosphate solubilizing ...

The highest alkaline phosphatase activity by A. faecalis was obtained at pH 9.0. Maximum alkaline phosphatase activity at pH 9.0 was also reported from Micrococcus sodonensis (Glew & Heath, 1971). An increase in temperature accelerates the velocity of an enzyme-catalyzed reaction until an optimum is reached, after which the velocity decreases ...

Optimization and production of Extracellular alkaline ...

phosphate mobilization to the plants. Alkaline phosphatase is commonly used as a tool in molecular biology and clinical assays7,8. Intracellular production of Alkaline phosphatase is quite tedious and expensive process in comparison to extracellular. The extracellular Alkaline phosphatase gave higher specific activity than intracellular Alkaline

DETERMINATION OF SERUM ACID AND ALKALINE …

use could be extended to the estimation of serum acid phosphatase activity. Considerable modifi-cation of the original Grifols procedure was found to be necessary in order to measure both alkaline and acid phosphatase activity in blood. The use of calibration curves, the stability of the colour, and the effect of the presence of serum on the ...

Comparison of several methods of estimating alkaline ...

Methods compared for estimation of alkaline phosphatase in human serum were those of King and Armstrong (Canad. Med. Assoc. J., 1934, 31, 376), Huggins and Talalay (J. Biol. Chem., 1945, 159, 399) and Sibata and Hasegava (reference to Russian abstract only). The last proved the most precise and could be used with only 0.03 ml serum. The substrate was that used by King and Armstrong, sodium...

The use of alkaline phosphatase and possible alternative ...

The use of alkaline phosphatase and possible alternative testing to verify pasteurisation of raw milk, colostrum, dairy ... verification of pasteurisation of the products immediately after such treatment in the processing plant, ... the respective species would facilitate an estimation of residual ALP after pasteurisation. However, only ...

Purification and Characterization of an Alkaline ...

With the exception of fructose 1,6 bi–phosphatase, sucrose 6– phosphate phosphatase [5] and an inducible alkaline phosphatase from Spirodela oligorrhiza [24], very few alkaline phosphatases are induced by Pi–deficiency. Plant alkaline phosphatases generally display rather strict substrate specificity and play defined roles in metabolism [10].

THE ESTIMATION OF PHOSPHATASE ACTIVITY IN SOIL

Soil phosphatase activities as affected by different crops in the same rotation The 2-crop rotation. Acid phosphatase activity measured in the wheat soil exceeded significantly (p 0.01) the coresponding activity recorded in the maize soil. Alkaline phosphatase activity is the same under wheat and maize crops. The 3-crop rotation. Significant (p ...

ISSN Print: Estimation and Comparison of Levels of ...

Alkaline Phosphatase 30 53 410 205.03 101.4 Acid Phosphatase 30 0.60 11 4.32 2.39 Calcium 30 3.5 30.2 13.29 7.03 Potassium 30 3 7.3 5.02 1.24 Table 2 revealed descriptive data of the study variables. Alkaline phophatase (205.03) and calcium (13.29) minerals showed highest mean value

RJPT - Estimation of Alkaline Phosphatase level in the ...

Background: Alkaline phosphatase (ALP) found on most cell membranes in the body. Hypothyroid patients tend to exhibit higher than normal bone density usually with normal or low ALP. Abnormal salivary ALP level reflects destructive processes in alveolar bone in periodontitis. Objectives: This study aims to measure the level of ALP in serum and saliva of hypothyroid patients with and …

Alkaline Phosphatase Test | ALP Test | ALP Blood Test

Test for estimation of Alkaline Phosphatase activity in serum / plasma using PNPP method. Introduction: Serum Alkaline Phosphatase Test levels are of interest in the diagnosis of hepatobiliary disorders and bone disease associated with increased osteoblastic activity. Moderate elevations of Alkaline Phosphatase may be seen in Hodgkin's ...

Antihyaluronidase and Alkaline Phosphatase (ALP ...

Syeda Fatima, Nazia Aslam, Sofia Khalid, Kalim Ullah, Khizar Abbas, Shahzad Hussain, Syed Sajid Hussain Shah, Zia-Ur-Rahman Qureshi, Mughal Qayum, Muhammad Hassham Hassan Bin Asad, " Antihyaluronidase and Alkaline Phosphatase (ALP) Activities of Medicinal Plants to Combat Echis carinatus Venom-Induced Toxicities ", BioMed Research International,. vol. 2021, Article ID …

ESTIMATION OF ALKALINE PHOSPHATASE AND PROTEIN …

Estimation of Alkaline phosphatase (ALP): Alkaline phosphatase was determined by the colorimetric kit method of Randox No. 307. One test tube was taken and add 0.02 ml of homogenate/supernatant and then 1ml of substrate was added and mixed. Reading was …

Veterinary Laboratory Diagnosis-II: Estimation of Alkaline ...

ESTIMATION OF SERUM ALKALINE PHOSPHATASE (p-NPP method – Kinetic mode) Principle: Alkaline phosphatase (ALP) hydrolyzes p-nitrophenyl phosphate (p-NPP) into p-nitrophenol and inorganic phosphate at pH 10.0. The p-nitrophenol is a yellow compound, which absorbs light at 405 nm. The rate of increase in absorbance at 405 nm is proportional to ...

PHYTIC ACID (PHYTATE)/ TOTAL PHOSPHORUS

Alkaline phosphatase will also release phosphate from monophosphate esters other than myo-inositol phosphate, however analyses of a broad spectrum of samples using K-PHYT indicated that phosphorus released from monophosphate esters other than myo-inositol phosphate is small.

Estimation of serum zinc and alkaline phosphatase in ...

The alkaline phosphatase activity was also lower in the malnourished children as compared to control group (p<0.001). There was a positive correlation between the alkaline phosphatase activity and the zinc concentration. The low serum zinc levels were also corre-lated positively with hypolbuminaemia.

WEEK #3: ENZYME KINETICS CHARACTERIZATION OF THE …

Alkaline phosphatase is a ubiquitous enzyme that can be isolated from bone, kidney, intestine, plasma, liver, spleen, plants and microorganisms. It catalyzes the removal of a phosphate that is attached to an alcohol, resulting in the generation of a free phosphate (Pi) and an alcohol.

Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369 ...

Alkaline Phosphatase Assay Kit (Colorimetric) ab83369 is a highly sensitive, simple, direct and HTS-ready colorimetric assay designed to measure alkaline phosphatase (ALP) activity in serum and other mammalian samples. The kit is a pNPP assay; it uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax= 405 nm ...

Estimation of Acid Phosphatase | Enzyme Activity

In this article we will discuss about the tests for estimation of acid phosphatase in plants. Principle: The enzyme phosphatase hydrolyzes p-nitro-phenol phosphate. The released p-nitro-phenol is yellow in colour in alkaline medium and is measured at 405 nm. The optimum pH for acid and alkaline phos­phatases are 5.3 and 10.5, respectively ...

PHOSPHATE SOLUBILIZATION POTENTIAL AND …

plant growth. Key words: Phosphate solubilization, Trichoderma, Acid phosphatase, Alkaline phosphatase, Chickpea. INTRODUCTION Phosphorus (P) is second only to Nitrogen (N) as the most limiting element for plant growth (3,18), making up about 0.2% of a plant's dry weight (25). The concentration of soluble

(PDF) Alkaline phosphatase in pleural effusions

In receiver operating characteristic curve analysis, sensitivity and specificity values were 90% and 80% for a cut-off value of 71 IU/L for pleural alkaline phosphatase activity; and were 90% and ...

Laboratory Procedure Manual

phosphate. This reaction occurs at an alkaline pH of 10.3. The system monitors the rate of change in absorbance at 410 nm over a fixed-time interval. This rate of change in absorbance is directly proportional to the ALP activity in the serum. Alkaline phosphatase measurements are used in the diagnosis and treatment of liver, bone, and parathyroid

Alkaline Phosphatase: An Overview

Introduction. Alkaline phosphatases [ALP; orthophosphoric monoester phosphohydrolase (alkaline optimum) EC 3.1.3.1] are plasma membrane-bound glycoproteins [1, 2].These enzymes are widely distributed in nature, including prokaryotes and higher eukaryotes [3–6], with the exception of some higher plants [].Alkaline phosphatase forms a large family of dimeric enzymes, usually …

ESTIMATION OF ALKALINE PHOSPHATASE AND PROTEIN …

Level of alkaline phosphatase and protein content were observed in Cotugnia Streptopell, Raillietina galeritae and Raillietina flaccida in whole cestode from domestic pigeons (Columba livia). Mean values of alkaline phosphatse in C. streptopell, R.galeritae and R. flaccida were observed as 22.908, 19.504, 19.228 U/l respectively, while mean values of protein content were observed as …

Research Article Effect of purified alkaline phosphatase ...

Sephadex G-200. Activity of alkaline phosphatase was estimated in each fraction and total protein was simultaneously determined by Bradford method. SDS-PAGE electrophoresis was used for estimation of molecular weight of purified extract of alkaline phosphatase. Characterization of alkaline Phosphatase Kinetic constant values Km and Vmax for ...